Авторы проанализировали данные 326 участников американского исследования NHANES, у которых в 1999–2000 годах брали образцы крови. Концентрации PFNA и PFOSA обнаружились примерно у 95 процентов обследованных. Затем ученые оценили биологический возраст добровольцев. Выяснилось, что более высокий уровень PFNA в крови мужчин 50–64 лет был связан с более быстрым эпигенетическим старением по ряду показателей. У женщин такой связи обнаружено не было.
The first version used Gopher,
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The year before, Nyren’s lab had also spun off a company, Pyrosequencing AB, to refine and commercialize the technology. Pyrosequencing was not the firm that would bring the technology to market, however; that distinction went to Connecticut-based 454 Life Sciences, who licensed whole-genome pyrosequencing applications in 2003. 454 made chips which enabled highly efficient, parallelized sequencing reactions and released the GS20 sequencer in 2005 for the price of $500,000. The GS20 worked by attaching each individual DNA template molecule to a bead and copying it many times using polymerase chain reaction (PCR). Each bead was then loaded into a well in a microplate, where sequencing reactions would be carried out. The light from luciferase activation could be detected through the bottom of the wells, enabling sequences to be read.